This application note describes how to create and generate standard-conform IEEE 802.15.4 test signals with the Rohde & Schwarz vector signal generators (VSG). It provides a brief introduction in the IEEE 802.15.4 PHY and MAC layer, explains in detail how a user-defined frame can be created by using the R&S IEEE 802.15.4 Frame Builder Excel spreadsheet and provides the information required to generate a Frame Builder based test signal with the Rohde & Schwarz vector signal generators. Finally an overview of standardized IEEE 802.15.4 PHY receiver tests is given.